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paav hsyn egfp vector  (Addgene inc)


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    Addgene inc paav hsyn egfp vector
    Paav Hsyn Egfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+hsyn+vector/pAAV-hSyn-EGFP+(Plasmid+%2350465)/pm41912102-306-1-4
    Average 96 stars, based on 375 article reviews
    paav hsyn egfp vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: A Critical Role of Presynaptic Cadherin/Catenin/p140Cap Complexes in Stabilizing Spines and Functional Synapses in the Neocortex.
    Article Snippet: .. pAAV-hSyn-GFP-b-cat* (AAV-GFP-b-cat* for short) encodes a stabilized form of b-catenin in which the GSK3b phosphorylation sites S33, S37, T41, S45 have beenmutated to alanines, resulting in a net effect similar to b-catlox(ex3); it was generated by subcloningGFPb-cat* (Yu and Malenka, 2003) into the pAAV-hSyn vector (human synapsin promoter, Addgene plasmid 26976). ..

    Phospho-proteomics:

    Article Title: A Critical Role of Presynaptic Cadherin/Catenin/p140Cap Complexes in Stabilizing Spines and Functional Synapses in the Neocortex.
    Article Snippet: .. pAAV-hSyn-GFP-b-cat* (AAV-GFP-b-cat* for short) encodes a stabilized form of b-catenin in which the GSK3b phosphorylation sites S33, S37, T41, S45 have beenmutated to alanines, resulting in a net effect similar to b-catlox(ex3); it was generated by subcloningGFPb-cat* (Yu and Malenka, 2003) into the pAAV-hSyn vector (human synapsin promoter, Addgene plasmid 26976). ..

    Generated:

    Article Title: A Critical Role of Presynaptic Cadherin/Catenin/p140Cap Complexes in Stabilizing Spines and Functional Synapses in the Neocortex.
    Article Snippet: .. pAAV-hSyn-GFP-b-cat* (AAV-GFP-b-cat* for short) encodes a stabilized form of b-catenin in which the GSK3b phosphorylation sites S33, S37, T41, S45 have beenmutated to alanines, resulting in a net effect similar to b-catlox(ex3); it was generated by subcloningGFPb-cat* (Yu and Malenka, 2003) into the pAAV-hSyn vector (human synapsin promoter, Addgene plasmid 26976). ..

    Plasmid Preparation:

    Article Title: A Critical Role of Presynaptic Cadherin/Catenin/p140Cap Complexes in Stabilizing Spines and Functional Synapses in the Neocortex.
    Article Snippet: .. pAAV-hSyn-GFP-b-cat* (AAV-GFP-b-cat* for short) encodes a stabilized form of b-catenin in which the GSK3b phosphorylation sites S33, S37, T41, S45 have beenmutated to alanines, resulting in a net effect similar to b-catlox(ex3); it was generated by subcloningGFPb-cat* (Yu and Malenka, 2003) into the pAAV-hSyn vector (human synapsin promoter, Addgene plasmid 26976). ..

    Article Title: Optogenetic Control of Receptor-mediated Growth Cone Dynamics in Neurons
    Article Snippet: Received: Jul 26, 2023 Revised: Dec 3, 2024 Accepted: Dec 10, 2024 ABSTRACT Development of neuronal connections is spatially and temporally controlled by extracellular cues which often activate their cognate cell surface receptors and elicit localized cellular responses.. Here, we demonstrate the use of an optogenetic tool to activate receptor signaling locally to induce actin-mediated growth cone remodeling in neurons.. Based on the light-induced interaction between Cryptochrome 2 (CRY2) and CIB1, we generated a bicistronic vector to co-expresses CRY2 fused to the intracellular domain of a guidance receptor and a membrane-anchored CIB1.



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    Addgene inc aav9 hsyn dio mcherry
    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent <t>AAV8-hSyn-DIO-hM4D(Gi)-mCherry</t> or <t>AAV8-hSyn-DIO-hM3D(Gq)-mCherry,</t> with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.
    Aav9 Hsyn Dio Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    virus strains aavrg hsyn egfp penn vector core addgene addgene  (Addgene inc)
    96
    Addgene inc virus strains aavrg hsyn egfp penn vector core addgene addgene
    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent <t>AAV8-hSyn-DIO-hM4D(Gi)-mCherry</t> or <t>AAV8-hSyn-DIO-hM3D(Gq)-mCherry,</t> with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.
    Virus Strains Aavrg Hsyn Egfp Penn Vector Core Addgene Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    aav1 aav1 syn chrimsonr tdtomato penn vector core addgene addgene  (Addgene inc)
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    Addgene inc aav1 aav1 syn chrimsonr tdtomato penn vector core addgene addgene
    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent <t>AAV8-hSyn-DIO-hM4D(Gi)-mCherry</t> or <t>AAV8-hSyn-DIO-hM3D(Gq)-mCherry,</t> with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.
    Aav1 Aav1 Syn Chrimsonr Tdtomato Penn Vector Core Addgene Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent AAV8-hSyn-DIO-hM4D(Gi)-mCherry or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.

    Journal: bioRxiv

    Article Title: External Globus Pallidus Arkypallidal Circuit Dynamics Gate Risk-Taking Behavior

    doi: 10.64898/2026.03.20.713182

    Figure Lengend Snippet: (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent AAV8-hSyn-DIO-hM4D(Gi)-mCherry or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.

    Article Snippet: The Gi/o-coupled DREADD vector AAV8-hSyn-DIO-hM4D(Gi)-mCherry (plasmid #44362) and the Gq-coupled DREADD vector AAV8-hSyn-DIO-hM3D(Gq)-mCherry (plasmid #44361) were obtained from Addgene.

    Techniques: Control

    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent AAV8-hSyn-DIO-hM4D(Gi)-mCherry or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.

    Journal: bioRxiv

    Article Title: External Globus Pallidus Arkypallidal Circuit Dynamics Gate Risk-Taking Behavior

    doi: 10.64898/2026.03.20.713182

    Figure Lengend Snippet: (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent AAV8-hSyn-DIO-hM4D(Gi)-mCherry or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.

    Article Snippet: The Gi/o-coupled DREADD vector AAV8-hSyn-DIO-hM4D(Gi)-mCherry (plasmid #44362) and the Gq-coupled DREADD vector AAV8-hSyn-DIO-hM3D(Gq)-mCherry (plasmid #44361) were obtained from Addgene.

    Techniques: Control